|
KMID : 0613820030130010099
|
|
Journal of Life Science
2003 Volume.13 No. 1 p.99 ~ p.104
|
|
|
Interaction of Phenolic Compound-Specific Activator with Its Promoter using SPR-Based DNA Chip
|
|
Park Sun-Mi
Park Hoo-Hwi
Lim Woon-Ki
Shin Hae-Ja
|
|
|
Abstract
|
|
|
Aromatic compounds are of major concern among environmental pollutants due to their toxicity and persistence. To monitor aromatic compounds in a real time with a better sensitivity, a new method of SPR (surface plasmon resonance) based on DNA chip (Biacore 3000) was developed here. It is thought that CapR regulatory protein as a complex with phenol, could bind to their corresponding promoter, Po. Biotinylated DNA oligomers for the promoter was synthesized by PCR and coupled onto streptoavidin-linked CM5-chip. CapR regulatory proteins were purified after
cloning their genes in pET21a (+) vector and expressing proteins. The interaction was assessed by the system where the regulatory proteins flowed with or without phenol through the cells of DNA chip. CapR regulatory protein even in the presence of phenol had no response to its promoter, Po, suggesting that other factor(s) might be required for the activation of Po promoter. The present work reveals a promising possibility of the SPR-based DNA chip in monitoring specific environmental pollutants in a real time.
|
|
|
KEYWORD
|
|
|
activator, SPR, phenol.
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
|